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smart seq2 library sequencing  (Novogene)
86
Novogene smart seq2 library sequencing
Increased chromatin accessibility in knockout round spermatids. A).Volcano plot of DEGs between control and Eif5a SKO samples.FDR<0.05. B.The average tag density plot(top pannel) and heatmaps (bottom pannel)around TSS (±3 kb) for the enrichment of ATAC‐seq reads in control and Eif5a SKO round spermatids. C).Plot shows Gain and Loss sites in all control and Eif5a SKO sample replicates. D).Venn diagram showing the overlap of differential peaks identified by CUT&Tag and ATAC‐seq between WT and CKO groups. E).Four‐quadrant scatter plot comparing the log 2 fold changes of significantly differential peaks (FDR < 0.05) from H3K4me3 CUT&Tag (x‐axis) and ATAC‐seq (y‐axis). The Pearson correlation coefficient for the compared data is 0.468. Pearson's *r* = 0.468. F) Venn diagram show shared genes between ATAC‐seq (FDR<0.05) <t>and</t> <t>Smart‐seq2</t> (FDR<0.05, FC>1.5).
Smart Seq2 Library Sequencing, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/smart seq2 library sequencing/product/Novogene
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smart seq2 library sequencing - by Bioz Stars, 2026-06
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smart-seq2 library sequencing  (Novogene)
90
Novogene smart-seq2 library sequencing
Increased chromatin accessibility in knockout round spermatids. A).Volcano plot of DEGs between control and Eif5a SKO samples.FDR<0.05. B.The average tag density plot(top pannel) and heatmaps (bottom pannel)around TSS (±3 kb) for the enrichment of ATAC‐seq reads in control and Eif5a SKO round spermatids. C).Plot shows Gain and Loss sites in all control and Eif5a SKO sample replicates. D).Venn diagram showing the overlap of differential peaks identified by CUT&Tag and ATAC‐seq between WT and CKO groups. E).Four‐quadrant scatter plot comparing the log 2 fold changes of significantly differential peaks (FDR < 0.05) from H3K4me3 CUT&Tag (x‐axis) and ATAC‐seq (y‐axis). The Pearson correlation coefficient for the compared data is 0.468. Pearson's *r* = 0.468. F) Venn diagram show shared genes between ATAC‐seq (FDR<0.05) <t>and</t> <t>Smart‐seq2</t> (FDR<0.05, FC>1.5).
Smart Seq2 Library Sequencing, supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/smart-seq2 library sequencing/product/Novogene
Average 90 stars, based on 1 article reviews
smart-seq2 library sequencing - by Bioz Stars, 2026-06
90/100 stars
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smart-seq2 libraries  (Illumina Inc)
90
Illumina Inc smart-seq2 libraries
Increased chromatin accessibility in knockout round spermatids. A).Volcano plot of DEGs between control and Eif5a SKO samples.FDR<0.05. B.The average tag density plot(top pannel) and heatmaps (bottom pannel)around TSS (±3 kb) for the enrichment of ATAC‐seq reads in control and Eif5a SKO round spermatids. C).Plot shows Gain and Loss sites in all control and Eif5a SKO sample replicates. D).Venn diagram showing the overlap of differential peaks identified by CUT&Tag and ATAC‐seq between WT and CKO groups. E).Four‐quadrant scatter plot comparing the log 2 fold changes of significantly differential peaks (FDR < 0.05) from H3K4me3 CUT&Tag (x‐axis) and ATAC‐seq (y‐axis). The Pearson correlation coefficient for the compared data is 0.468. Pearson's *r* = 0.468. F) Venn diagram show shared genes between ATAC‐seq (FDR<0.05) <t>and</t> <t>Smart‐seq2</t> (FDR<0.05, FC>1.5).
Smart Seq2 Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/smart-seq2 libraries/product/Illumina Inc
Average 90 stars, based on 1 article reviews
smart-seq2 libraries - by Bioz Stars, 2026-06
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smart-seq2 library preparation kit  (Nextera AS)
90
Nextera AS smart-seq2 library preparation kit
Increased chromatin accessibility in knockout round spermatids. A).Volcano plot of DEGs between control and Eif5a SKO samples.FDR<0.05. B.The average tag density plot(top pannel) and heatmaps (bottom pannel)around TSS (±3 kb) for the enrichment of ATAC‐seq reads in control and Eif5a SKO round spermatids. C).Plot shows Gain and Loss sites in all control and Eif5a SKO sample replicates. D).Venn diagram showing the overlap of differential peaks identified by CUT&Tag and ATAC‐seq between WT and CKO groups. E).Four‐quadrant scatter plot comparing the log 2 fold changes of significantly differential peaks (FDR < 0.05) from H3K4me3 CUT&Tag (x‐axis) and ATAC‐seq (y‐axis). The Pearson correlation coefficient for the compared data is 0.468. Pearson's *r* = 0.468. F) Venn diagram show shared genes between ATAC‐seq (FDR<0.05) <t>and</t> <t>Smart‐seq2</t> (FDR<0.05, FC>1.5).
Smart Seq2 Library Preparation Kit, supplied by Nextera AS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/smart-seq2 library preparation kit/product/Nextera AS
Average 90 stars, based on 1 article reviews
smart-seq2 library preparation kit - by Bioz Stars, 2026-06
90/100 stars
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smart-seq2 library preparation  (Illumina Inc)
90
Illumina Inc smart-seq2 library preparation
Increased chromatin accessibility in knockout round spermatids. A).Volcano plot of DEGs between control and Eif5a SKO samples.FDR<0.05. B.The average tag density plot(top pannel) and heatmaps (bottom pannel)around TSS (±3 kb) for the enrichment of ATAC‐seq reads in control and Eif5a SKO round spermatids. C).Plot shows Gain and Loss sites in all control and Eif5a SKO sample replicates. D).Venn diagram showing the overlap of differential peaks identified by CUT&Tag and ATAC‐seq between WT and CKO groups. E).Four‐quadrant scatter plot comparing the log 2 fold changes of significantly differential peaks (FDR < 0.05) from H3K4me3 CUT&Tag (x‐axis) and ATAC‐seq (y‐axis). The Pearson correlation coefficient for the compared data is 0.468. Pearson's *r* = 0.468. F) Venn diagram show shared genes between ATAC‐seq (FDR<0.05) <t>and</t> <t>Smart‐seq2</t> (FDR<0.05, FC>1.5).
Smart Seq2 Library Preparation, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/smart-seq2 library preparation/product/Illumina Inc
Average 90 stars, based on 1 article reviews
smart-seq2 library preparation - by Bioz Stars, 2026-06
90/100 stars
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vahts mrnaseq v3 library prep kit for illumina smart-seq2  (Illumina Inc)
90
Illumina Inc vahts mrnaseq v3 library prep kit for illumina smart-seq2
Increased chromatin accessibility in knockout round spermatids. A).Volcano plot of DEGs between control and Eif5a SKO samples.FDR<0.05. B.The average tag density plot(top pannel) and heatmaps (bottom pannel)around TSS (±3 kb) for the enrichment of ATAC‐seq reads in control and Eif5a SKO round spermatids. C).Plot shows Gain and Loss sites in all control and Eif5a SKO sample replicates. D).Venn diagram showing the overlap of differential peaks identified by CUT&Tag and ATAC‐seq between WT and CKO groups. E).Four‐quadrant scatter plot comparing the log 2 fold changes of significantly differential peaks (FDR < 0.05) from H3K4me3 CUT&Tag (x‐axis) and ATAC‐seq (y‐axis). The Pearson correlation coefficient for the compared data is 0.468. Pearson's *r* = 0.468. F) Venn diagram show shared genes between ATAC‐seq (FDR<0.05) <t>and</t> <t>Smart‐seq2</t> (FDR<0.05, FC>1.5).
Vahts Mrnaseq V3 Library Prep Kit For Illumina Smart Seq2, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vahts mrnaseq v3 library prep kit for illumina smart-seq2/product/Illumina Inc
Average 90 stars, based on 1 article reviews
vahts mrnaseq v3 library prep kit for illumina smart-seq2 - by Bioz Stars, 2026-06
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vahts mrna-seq v3 library prep kit for illumina smart-seq2  (Illumina Inc)
90
Illumina Inc vahts mrna-seq v3 library prep kit for illumina smart-seq2
Increased chromatin accessibility in knockout round spermatids. A).Volcano plot of DEGs between control and Eif5a SKO samples.FDR<0.05. B.The average tag density plot(top pannel) and heatmaps (bottom pannel)around TSS (±3 kb) for the enrichment of ATAC‐seq reads in control and Eif5a SKO round spermatids. C).Plot shows Gain and Loss sites in all control and Eif5a SKO sample replicates. D).Venn diagram showing the overlap of differential peaks identified by CUT&Tag and ATAC‐seq between WT and CKO groups. E).Four‐quadrant scatter plot comparing the log 2 fold changes of significantly differential peaks (FDR < 0.05) from H3K4me3 CUT&Tag (x‐axis) and ATAC‐seq (y‐axis). The Pearson correlation coefficient for the compared data is 0.468. Pearson's *r* = 0.468. F) Venn diagram show shared genes between ATAC‐seq (FDR<0.05) <t>and</t> <t>Smart‐seq2</t> (FDR<0.05, FC>1.5).
Vahts Mrna Seq V3 Library Prep Kit For Illumina Smart Seq2, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vahts mrna-seq v3 library prep kit for illumina smart-seq2/product/Illumina Inc
Average 90 stars, based on 1 article reviews
vahts mrna-seq v3 library prep kit for illumina smart-seq2 - by Bioz Stars, 2026-06
90/100 stars
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smart-seq2 transcriptome libraries  (Broad Institute Inc)
90
Broad Institute Inc smart-seq2 transcriptome libraries
Increased chromatin accessibility in knockout round spermatids. A).Volcano plot of DEGs between control and Eif5a SKO samples.FDR<0.05. B.The average tag density plot(top pannel) and heatmaps (bottom pannel)around TSS (±3 kb) for the enrichment of ATAC‐seq reads in control and Eif5a SKO round spermatids. C).Plot shows Gain and Loss sites in all control and Eif5a SKO sample replicates. D).Venn diagram showing the overlap of differential peaks identified by CUT&Tag and ATAC‐seq between WT and CKO groups. E).Four‐quadrant scatter plot comparing the log 2 fold changes of significantly differential peaks (FDR < 0.05) from H3K4me3 CUT&Tag (x‐axis) and ATAC‐seq (y‐axis). The Pearson correlation coefficient for the compared data is 0.468. Pearson's *r* = 0.468. F) Venn diagram show shared genes between ATAC‐seq (FDR<0.05) <t>and</t> <t>Smart‐seq2</t> (FDR<0.05, FC>1.5).
Smart Seq2 Transcriptome Libraries, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/smart-seq2 transcriptome libraries/product/Broad Institute Inc
Average 90 stars, based on 1 article reviews
smart-seq2 transcriptome libraries - by Bioz Stars, 2026-06
90/100 stars
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smart-seq2 libraries  (Broad Technology Labs)
90
Broad Technology Labs smart-seq2 libraries
Increased chromatin accessibility in knockout round spermatids. A).Volcano plot of DEGs between control and Eif5a SKO samples.FDR<0.05. B.The average tag density plot(top pannel) and heatmaps (bottom pannel)around TSS (±3 kb) for the enrichment of ATAC‐seq reads in control and Eif5a SKO round spermatids. C).Plot shows Gain and Loss sites in all control and Eif5a SKO sample replicates. D).Venn diagram showing the overlap of differential peaks identified by CUT&Tag and ATAC‐seq between WT and CKO groups. E).Four‐quadrant scatter plot comparing the log 2 fold changes of significantly differential peaks (FDR < 0.05) from H3K4me3 CUT&Tag (x‐axis) and ATAC‐seq (y‐axis). The Pearson correlation coefficient for the compared data is 0.468. Pearson's *r* = 0.468. F) Venn diagram show shared genes between ATAC‐seq (FDR<0.05) <t>and</t> <t>Smart‐seq2</t> (FDR<0.05, FC>1.5).
Smart Seq2 Libraries, supplied by Broad Technology Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/smart-seq2 libraries/product/Broad Technology Labs
Average 90 stars, based on 1 article reviews
smart-seq2 libraries - by Bioz Stars, 2026-06
90/100 stars
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Image Search Results


Increased chromatin accessibility in knockout round spermatids. A).Volcano plot of DEGs between control and Eif5a SKO samples.FDR<0.05. B.The average tag density plot(top pannel) and heatmaps (bottom pannel)around TSS (±3 kb) for the enrichment of ATAC‐seq reads in control and Eif5a SKO round spermatids. C).Plot shows Gain and Loss sites in all control and Eif5a SKO sample replicates. D).Venn diagram showing the overlap of differential peaks identified by CUT&Tag and ATAC‐seq between WT and CKO groups. E).Four‐quadrant scatter plot comparing the log 2 fold changes of significantly differential peaks (FDR < 0.05) from H3K4me3 CUT&Tag (x‐axis) and ATAC‐seq (y‐axis). The Pearson correlation coefficient for the compared data is 0.468. Pearson's *r* = 0.468. F) Venn diagram show shared genes between ATAC‐seq (FDR<0.05) and Smart‐seq2 (FDR<0.05, FC>1.5).

Journal: Advanced Science

Article Title: EIF5A Couples Translational Control With Transcriptional Reprogramming Through Chromocenter Reorganization During Spermiogenesis

doi: 10.1002/advs.202517423

Figure Lengend Snippet: Increased chromatin accessibility in knockout round spermatids. A).Volcano plot of DEGs between control and Eif5a SKO samples.FDR<0.05. B.The average tag density plot(top pannel) and heatmaps (bottom pannel)around TSS (±3 kb) for the enrichment of ATAC‐seq reads in control and Eif5a SKO round spermatids. C).Plot shows Gain and Loss sites in all control and Eif5a SKO sample replicates. D).Venn diagram showing the overlap of differential peaks identified by CUT&Tag and ATAC‐seq between WT and CKO groups. E).Four‐quadrant scatter plot comparing the log 2 fold changes of significantly differential peaks (FDR < 0.05) from H3K4me3 CUT&Tag (x‐axis) and ATAC‐seq (y‐axis). The Pearson correlation coefficient for the compared data is 0.468. Pearson's *r* = 0.468. F) Venn diagram show shared genes between ATAC‐seq (FDR<0.05) and Smart‐seq2 (FDR<0.05, FC>1.5).

Article Snippet: The Smart‐seq2 library sequencing was performed by Novogene on Illumina platforms, generating 150 bp paired‐end reads.

Techniques: Knock-Out, Control

Proteomic alterations associated with transcriptional changes induced by Eif5a deletion. A).Venn diagram of shared genes between Smart‐seq2 (P value<0.05, FC>1.5) and Proteomics (P<0.05, FC>1.5) analyses. B).GO enrichment analysis based on the 119 commonly upregulated genes. C).QRT‐PCR analysis of candidate genes that were consistently dysregulated in both the transcriptome and proteome of Eif5a SKO testes. Data are presented as mean ± SD from three independent biological replicates (n = 3). Statistical significance was determined using a two‐tailed, unpaired Student's t‐test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). D). Western blots show SPATA1, SPACA3 and SPACA9 proteins in Eif5a SKO and control mice. β‐Actin served as the loading control. E). IGV visualization of genomic regions harboring acrosome‐related ( Spaca3, Ly6K,Spaca9,Spata1,Lamp2 ) and microtubule‐associated ( Ccdc169, Dynlt3 ) genes. Top: ATAC‐seq tracks showing chromatin accessibility in control (blue) versus SKO (red) round spermatids. Bottom: Corresponding Smart‐seq2 coverage.

Journal: Advanced Science

Article Title: EIF5A Couples Translational Control With Transcriptional Reprogramming Through Chromocenter Reorganization During Spermiogenesis

doi: 10.1002/advs.202517423

Figure Lengend Snippet: Proteomic alterations associated with transcriptional changes induced by Eif5a deletion. A).Venn diagram of shared genes between Smart‐seq2 (P value<0.05, FC>1.5) and Proteomics (P<0.05, FC>1.5) analyses. B).GO enrichment analysis based on the 119 commonly upregulated genes. C).QRT‐PCR analysis of candidate genes that were consistently dysregulated in both the transcriptome and proteome of Eif5a SKO testes. Data are presented as mean ± SD from three independent biological replicates (n = 3). Statistical significance was determined using a two‐tailed, unpaired Student's t‐test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). D). Western blots show SPATA1, SPACA3 and SPACA9 proteins in Eif5a SKO and control mice. β‐Actin served as the loading control. E). IGV visualization of genomic regions harboring acrosome‐related ( Spaca3, Ly6K,Spaca9,Spata1,Lamp2 ) and microtubule‐associated ( Ccdc169, Dynlt3 ) genes. Top: ATAC‐seq tracks showing chromatin accessibility in control (blue) versus SKO (red) round spermatids. Bottom: Corresponding Smart‐seq2 coverage.

Article Snippet: The Smart‐seq2 library sequencing was performed by Novogene on Illumina platforms, generating 150 bp paired‐end reads.

Techniques: Quantitative RT-PCR, Two Tailed Test, Western Blot, Control